Allelotype of Renal Cell Carcinoma1
نویسندگان
چکیده
Several recent studies based on restriction fragment length polymor phism analysis have supported the concept that the accumulation of multiple genetic alterations converts a normal cell to a malignant cell. Activation of oncogenes and/or inactivation of tumor suppressor genes have been observed during tumor progression in colorectal cancer, lung cancer, and breast cancer. To investigate the possibility that multiple genes are altered during the progression of renal cell carcinoma, we have used restriction fragment length polymorphism markers throughout the genome to test for loss of heterozygosity in 38 renal cell carcinomas. Nearly 64% of the tumors had lost heterozygosity on the short arm of chromosome 3. We also observed loss of heterozygosity averaging about 30% at informative loci on six other chromosomal arms (chromosomes 5q, 6q, lOq, llq, 17p, and 19p). These results lead us to suspect the existence of several tumor suppressor genes associated with carcinogenesis of renal cell carcinoma.
منابع مشابه
Distal nephron renal tumors: microsatellite allelotype.
Tumors of varying malignant potential arise from the complex epithelial lining of the nephron. Although the molecular characteristics of renal clear cell carcinomas, which arise from the proximal tubule, have been studied, little is known about tumors that develop from other parts of the renal tubular system. To elucidate common molecular lesions that may contribute to the development or progre...
متن کاملAllelotype Analysis of Cervical Carcinoma1
To identify the genetic events which may play a role in the development of cervical carcinoma, we performed a detailed allelotype analysis utilizing DNA from 53 primary tumors and corresponding normal cells and 57 polymorphic probes mapped to each of the chromosomal arms, excluding the short arms of the acrocentric chromosomes. Loss of heterozygosity (LOH) of >25% was observed at sites on 11 ch...
متن کاملSmoking and Renal Cell Carcinoma1
The relationship between smoking habits and the risk of renal cell carcinoma was investigated in a case-control study conducted in northern Italy on 131 cases of histologically confirmed cancers of the renal parenchyma (85 males, 46 females) and 394 controls in hospital for acute, nonneoplastic, nonurological disorders. Compared with never smokers, the relative risk (RR) was 1.7 (95% confidence...
متن کاملGenetic Aberrations Detected by Comparative Genomic Hybridization Are Associated with Clinical Outcome in Renal Cell Carcinoma1
The clinical behavior of renal cell carcinoma (RCC) cannot be pre dicted by histological and other markers. In this study, comparative genomic hybridization was used to evaluate whether the number of genomic aberrations has prognostic significance in 41 nonmetastatic clear cell RCC extending beyond the renal capsule. Losses were most prevalent at 3p (56%) and 9p and 13q (24% each). The number o...
متن کاملAdvances in Brief Mutation, Allelotyping, and Transcription Analyses of the p73 Gene in Prostatic Carcinoma1
A novel gene,/? 73, encoding a protein with significant homology top53, was recently identified at Ip36. To investigate penetrance of p 73 in prostatic carcinogenesis, mutation, allelotyping, and transcription analy ses of p73 were performed in prostatic carcinoma. No types of mutation causing amino acid substitutions or frameshifts were found in 106 cases examined. Loss of heterozygosity in th...
متن کامل